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Product Info Summary
| SKU: | M30973 |
|---|---|
| Size: | 100μl/vial |
| Reactive Species: | All species |
| Host: | Mouse |
| Application: | Dot blot, ELISA, WB |
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Product info
Product Name
Anti-GST Tag Antibody (monoclonal, 5A7)
SKU/Catalog Number
M30973
Size
100μl/vial
Form
Liquid
Description
Boster Bio Anti-GST Tag Antibody (monoclonal, 5A7) catalog # M30973. Tested in Dot blot, ELISA, WB applications. This antibody reacts with All species.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GST Tag Antibody (monoclonal, 5A7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30973)
Host
Mouse
Contents
0.5mg/ml in PBS, pH7.4, containing and 50% glycerol.
Clonality
Monoclonal
Clone Number
5A7
Isotype
IgG2b
Immunogen
This antibody is produced by immunizing mouse with recombinant GST protein expressed in E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M30973 is reactive to Tmem177 in All species
Applications
M30973 is guaranteed for Dot blot, ELISA, WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
34.067kDa
Background of Tmem177
A GST-Tag is often used to separate and purify proteins that contain the GST-fusion protein. The Tag is 220 amino acids (roughly 26KDa), in size is quite large. It is fused to the N-terminus of a protein. However, many commercially available sources of GST-Tagged plasmids include a thrombin domain for cleavage of the GST Tag during protein purification.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
1:500-1:5000
Validation Images & Assay Conditions
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Western blot analysis of different concentration of Anti-GST Tag (5A7) Monoclonal Antibody
Antigen: Extracts from E.coli expressing GST-tagged protein
Line No.
1: 1 ug/mL
2: 0.5 ug/mL
3: 0.1 ug/mL
Blocking: Incubate the membrane with 5% Non-Fat Milk, PBS, 4 ℃ overnight
Antibody Incubation: Diluted antibody in 5% Non-Fat Milk, TBS, 0.1% Tween-20, RT, 30mins
Protein Target Info & Infographic
Gene/Protein Information For Tmem177 (Source: Uniprot.org, NCBI)
Gene Name
Tmem177
Full Name
Transmembrane protein 177
Weight
34.067kDa
Superfamily
TMEM177 family
Alternative Names
MGC10993; transmembrane protein 177
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tmem177, check out the Tmem177 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tmem177: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GST Tag Antibody (monoclonal, 5A7) (M30973)
Hello CJ!
M30973 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression and characterization of an esterase belonging to a new family via isolation from a metagenomic library of paper mill sludge
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Customer Reviews
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